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Chapters
- 00:00Title
- 00:10Introduction
- 01:06Cardiac Perfusion
- 05:36Removing the Brain
- 09:11Removing the Spinal Cord
- 11:18Preparation of a Single-Cell Suspension
- 14:12Percoll Gradient
- 17:41Harvest of Mononuclear Cells
- 19:56Conclusion
Tags
IsolationMononuclear CellsCentral Nervous SystemRatsEAEAutoimmune DiseaseImmune CellsInfectionPoliomyelitisLyme NeuroborreliosisNeurosyphilisVideo-protocolCardiac PerfusionSaline SolutionBlood ContaminationBrainSpinal CordDilacerationSingle-cell SuspensionPercoll GradientElectrophysiologyFlow Cytometry (FACS)BiochemistrySterile Conditions
