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Biology
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测量去泛素酶的活性细胞系和组织样品的方法
JoVE Journal
Biology
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JoVE Journal
Biology
Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples
Please note that all translations are automatically generated.
Click here for the English version.
测量去泛素酶的活性细胞系和组织样品的方法
DOI:
10.3791/52784-v
•
09:45 min
•
May 10, 2015
•
Percy Griffin
2
,
Ashley Sexton
,
Lauren Macneill
,
Yoshie Iizuka
4
,
Michael K. Lee
2
,
Martina Bazzaro
4
1
Department of Neuroscience
,
University of Minnesota
,
2
Institute for Translational Neuroscience
,
University of Minnesota
,
3
Department of Obstetrics, Gynecology, and Women’s Heath
,
University of Minnesota
,
4
Masonic Cancer Center
,
University of Minnesota
Chapters
00:05
Title
01:09
Culturing Cells for the Experiment
02:24
Cell Harvesting and Cell Lysis
04:40
Sample Derivatization
06:03
Western Blot
07:42
Results: Analysis of Deubiquinating Enzyme Activity
09:07
Conclusion
Summary
Automatic Translation
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Automatic Translation
目前的协议细节,用于测量功能性同源去泛素酶的活性的方法。专门探针共价修饰的酶,并允许进行检测。这种方法保存,以确定新的治疗靶点的潜力。
Tags
Keywords: Deubiquitinating Enzymes
Ubiquitin-proteasome System
Cell Lines
Activity Assay
Proteasomal Degradation
Active Site Probes
Enzyme Activity
Expression
Cell Disruption
Pathologies
Neurodegenerative Diseases
Cancer
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