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Metode til måling af aktiviteten af ​​afubiquitinerende Enzymer i cellelinier og vævsprøver
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JoVE Journal Biology
Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

Metode til måling af aktiviteten af ​​afubiquitinerende Enzymer i cellelinier og vævsprøver

DOI:
10.3791/52784-v

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09:45 min

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May 10, 2015

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, , , , ,
1Department of Neuroscience,University of Minnesota, 2Institute for Translational Neuroscience,University of Minnesota, 3Department of Obstetrics, Gynecology, and Women’s Heath,University of Minnesota, 4Masonic Cancer Center,University of Minnesota

Chapters

  • 00:05Title
  • 01:09Culturing Cells for the Experiment
  • 02:24Cell Harvesting and Cell Lysis
  • 04:40Sample Derivatization
  • 06:03Western Blot
  • 07:42Results: Analysis of Deubiquinating Enzyme Activity
  • 09:07Conclusion

Summary

Automatic Translation

Automatic Translation

Den nuværende protokol detaljer en fremgangsmåde til måling af aktiviteten af ​​funktionelt homologe afubiquitinerende enzymer. Specialiserede sonder kovalent modificere enzymet og give mulighed for detektion. Denne metode har potentiale til at identificere nye terapeutiske mål.

Tags

Keywords: Deubiquitinating EnzymesUbiquitin-proteasome SystemCell LinesActivity AssayProteasomal DegradationActive Site ProbesEnzyme ActivityExpressionCell DisruptionPathologiesNeurodegenerative DiseasesCancer

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