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Неинвазивная Визуализация врожденного иммунного ответа в данио рерио личинок модели<em> Streptococcus iniae</em> Инфекция
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Non-invasive Imaging of the Innate Immune Response in a Zebrafish Larval Model of Streptococcus iniae Infection
DOI:

11:16 min

April 21, 2015

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Chapters

  • 00:05Title
  • 01:23Preparation of Larval Injection Dishes
  • 02:31Otic Vesicle Injection of Streptococcus iniae in Three Day Old Larvae
  • 06:19Preparation of Larvae for Live Imaging
  • 07:34Photoconversion of Dendra2-labeled Leukocytes at the Otic Vesicle
  • 08:56Results: Streptococcus iniae Infection in the Zebrafish Larval Otic Vesicle
  • 10:28Conclusion

Summary

Automatic Translation

Here, we present a protocol for the generation and imaging of a localized bacterial infection in the zebrafish otic vesicle.

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