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从中央与EAE大鼠中枢神经系统的单核细胞的分离
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Isolation of Mononuclear Cells from the Central Nervous System of Rats with EAE
Please note that all translations are automatically generated.
Click here for the English version.
从中央与EAE大鼠中枢神经系统的单核细胞的分离
DOI:
10.3791/527-v
•
20:51 min
•
December 04, 2007
•
Christine Beeton
,
K. George Chandy
1
Department of Physiology and Biophysics
,
University of California, Irvine (UCI)
Chapters
00:00
Title
00:10
Introduction
01:06
Cardiac Perfusion
05:36
Removing the Brain
09:11
Removing the Spinal Cord
11:18
Preparation of a Single-Cell Suspension
14:12
Percoll Gradient
17:41
Harvest of Mononuclear Cells
19:56
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这个视频中,我们将演示如何从中央与实验性自身免疫性脑脊髓炎大鼠神经系统中分离单个核细胞。
Tags
Isolation
Mononuclear Cells
Central Nervous System
Rats
EAE
Autoimmune Disease
Immune Cells
Infection
Poliomyelitis
Lyme Neuroborreliosis
Neurosyphilis
Video-protocol
Cardiac Perfusion
Saline Solution
Blood Contamination
Brain
Spinal Cord
Dilaceration
Single-cell Suspension
Percoll Gradient
Electrophysiology
Flow Cytometry (FACS)
Biochemistry
Sterile Conditions
Article
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