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Lusiferaz haberci geni ile RAW264.7 hücreleri transfekte
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JoVE Journal Biology
Transfecting RAW264.7 Cells with a Luciferase Reporter Gene
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Lusiferaz haberci geni ile RAW264.7 hücreleri transfekte

DOI:
10.3791/52807-v

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12:58 min

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June 18, 2015

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, , ,
1Immunity and Infection Research Centre,Vancouver Costal Health Research Institute, 2Department of Surgery,University of British Columbia, 3Department of Biochemistry and Molecular Biology,University of British Columbia

Chapters

  • 00:05Title
  • 01:28Plasmid DNA Purification
  • 04:08Cell Culturing and Seeding
  • 05:06Lipid-based Transfection
  • 06:50Protein/Polyamine-based Transfection
  • 08:30Cell Stimulation and Luciferase Assay
  • 10:02Results: Macrophages are Transfected with Luciferase Reporter Genes without Affecting Cell Physiology
  • 12:25Conclusion

Summary

Automatic Translation

Automatic Translation

Transfection into the macrophage cell line, RAW264.7, is difficult due to the cell’s natural response against foreign materials. We described here a gentle yet robust procedure for transfecting luciferase reporter genes into RAW264.7 cells.

Tags

Luciferase ReporterTransfectionRAW264.7 Macrophage Cell LineLipid-based Transfection ReagentPolyamine-based Transfection ReagentTransfection EfficiencyBiomedical Research

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