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Live-Imaging af ependymale Cilia i laterale ventrikler af Mouse Brain
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JoVE Journal Neuroscience
Live Imaging of the Ependymal Cilia in the Lateral Ventricles of the Mouse Brain

Live-Imaging af ependymale Cilia i laterale ventrikler af Mouse Brain

DOI:
10.3791/52853-v

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06:58 min

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June 01, 2015

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, , , ,
1Department of Pharmacology and Experimental Therapeutics,University of Toledo, College of Pharmacy and Pharmaceutical Sciences, 2Life Sciences Institute,University of Michigan, 3Department of Biomedical & Pharmaceutical Sciences,Chapman University, School of Pharmacy, Rinker Health Science campus

Chapters

  • 00:05Title
  • 01:31Brain Extraction, Sectioning, and Tissue Preparation
  • 02:28Live Imaging Configuration and Setup
  • 04:00Calcium Signal Recording and Immunofluorescence Microscopy
  • 05:26Results: Measuring Ependymal Cilia Function and Calcium Signaling
  • 06:22Conclusion

Summary

Automatic Translation

Automatic Translation

Ved hjælp af høj opløsning kontrast differential interferens (DIC) mikroskopi er en ex vivo observation af det bankende af motile ependymale cilier beliggende inden for de mus hjerneventriklerne demonstreret ved levende billeddannelse. Teknikken tillader en optagelse af den unikke ciliær slagfrekvens og slå vinkel samt deres intracellulære calcium svingning pacing egenskaber.

Tags

Live ImagingEpendymal CiliaLateral VentriclesMouse BrainMulticiliated Ependymal CellsCiliary DynamicsCiliary Beating FrequencyCalcium OscillationsImmunofluorescence ImagingNeurological Deficits

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