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Neuroscience
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室管膜纤毛在小鼠脑侧脑室实时成像
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Live Imaging of the Ependymal Cilia in the Lateral Ventricles of the Mouse Brain
Please note that all translations are automatically generated.
Click here for the English version.
室管膜纤毛在小鼠脑侧脑室实时成像
DOI:
10.3791/52853-v
•
06:58 min
•
June 01, 2015
•
Alzahra J. Al Omran
,
Hannah C. Saternos
,
Tongyu Liu
,
Surya M. Nauli
,
Wissam A. AbouAlaiwi
1
Department of Pharmacology and Experimental Therapeutics
,
University of Toledo, College of Pharmacy and Pharmaceutical Sciences
,
2
Life Sciences Institute
,
University of Michigan
,
3
Department of Biomedical & Pharmaceutical Sciences
,
Chapman University, School of Pharmacy, Rinker Health Science campus
Chapters
00:05
Title
01:31
Brain Extraction, Sectioning, and Tissue Preparation
02:28
Live Imaging Configuration and Setup
04:00
Calcium Signal Recording and Immunofluorescence Microscopy
05:26
Results: Measuring Ependymal Cilia Function and Calcium Signaling
06:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
使用高分辨率微分干涉对比(DIC)显微镜,
离体
观察位于小鼠脑室内游动室管膜纤毛的跳动的证明通过实时成像。该技术允许独特纤毛跳动频率的记录和跳动角度以及它们的细胞内钙振荡起搏属性。
Tags
Live Imaging
Ependymal Cilia
Lateral Ventricles
Mouse Brain
Multiciliated Ependymal Cells
Ciliary Dynamics
Ciliary Beating Frequency
Calcium Oscillations
Immunofluorescence Imaging
Neurological Deficits
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