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的内肌浆网钙光学标测<sup> 2+</sup>和的Langendorff灌注兔心脏跨膜电位
JoVE Journal
Biology
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JoVE Journal Biology
Optical Mapping of Intra-Sarcoplasmic Reticulum Ca2+ and Transmembrane Potential in the Langendorff-perfused Rabbit Heart
DOI:

09:26 min

September 10, 2015

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Chapters

  • 00:05Title
  • 01:49Preparations
  • 03:31Harvesting, Perfusing and Dye-loading a Rabbit Heart
  • 07:09Optical Mapping
  • 07:56Resulting Vm and SR Ca2+ Signals from a Langendorff-perfused Rabbit Heart
  • 08:59Conclusion

Summary

Automatic Translation

本文介绍了详细的协议和必要的跨膜电位(V M)双光学测绘和免费内部肌浆网(SR)设备在的Langendorff灌注兔心脏。此方法允许直接观察和第V 量化和SR Ca 2+动力学在完整心脏。

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