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的内肌浆网钙光学标测<sup> 2+</sup>和的Langendorff灌注兔心脏跨膜电位
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Optical Mapping of Intra-Sarcoplasmic Reticulum Ca2+ and Transmembrane Potential in the Langendorff-perfused Rabbit Heart
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的内肌浆网钙光学标测<sup> 2+</sup>和的Langendorff灌注兔心脏跨膜电位

DOI:
10.3791/53166-v

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09:26 min

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September 10, 2015

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, ,
1Department of Pharmacology,University of California Davis, 2Departments of Pharmacology and Biomedical Engineering,University of California Davis

Chapters

  • 00:05Title
  • 01:49Preparations
  • 03:31Harvesting, Perfusing and Dye-loading a Rabbit Heart
  • 07:09Optical Mapping
  • 07:56Resulting Vm and SR Ca2+ Signals from a Langendorff-perfused Rabbit Heart
  • 08:59Conclusion

Summary

Automatic Translation

Automatic Translation

本文介绍了详细的协议和必要的跨膜电位（V M）双光学测绘和免费内部肌浆网（SR）设备钙在的Langendorff灌注兔心脏。此方法允许直接观察和第V 米量化和SR 的 Ca 2+动力学在完整心脏。

Tags

Optical MappingSarcoplasmic ReticulumCa2+ HandlingTransmembrane PotentialLangendorff-perfused HeartFluo-5NExcitation-contraction CouplingArrhythmiaSpatial MappingDual Optical Mapping

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