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In Vitro geração de células dendríticas plasmocitóides Murino de comum Linfóide Progenitores usando o sistema de alimentação AC-6
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
In Vitro Generation of Murine Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors using the AC-6 Feeder System
DOI:

08:18 min

November 23, 2015

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Chapters

  • 00:05Title
  • 01:03Bone Marrow Cell Isolation
  • 03:25Common Lymphoid Progenitor (CLP) Co-culture and Flow Cytometric Analysis
  • 06:01Results: Representative Murine CLP-derived pDC
  • 07:28Conclusion

Summary

Automatic Translation

In this study we present an in vitro culture system that can efficiently generate pDCs by co-culturing common lymphoid progenitors with AC-6 feeder cells in the presence of Flt3 ligand.

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