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Metoder för Exakt Lokaliserad Överföring av celler eller DNA i tidig postimplantationsförlust musembryon
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Methods for Precisely Localized Transfer of Cells or DNA into Early Postimplantation Mouse Embryos

Metoder för Exakt Lokaliserad Överföring av celler eller DNA i tidig postimplantationsförlust musembryon

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09:04 min

December 25, 2015

DOI:

09:04 min
December 25, 2015

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Summary

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We demonstrate a method for grafting cultured cells into defined sites of early mouse embryos to determine their in vivo potential. We also introduce an optimized electroporation method that uses glass capillaries of known diameter, allowing the precise delivery of exogenous DNA into a few cells in the embryos.

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