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使用荧光显微镜驱动蛋白-1的货物的识别
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Identification of Kinesin-1 Cargos Using Fluorescence Microscopy
Please note that all translations are automatically generated.
Click here for the English version.
使用荧光显微镜驱动蛋白-1的货物的识别
DOI:
10.3791/53632-v
•
08:06 min
•
February 14, 2016
•
Clement M. Lee
1
Department of Oncological Sciences
,
Icahn School of Medicine at Mount Sinai
Chapters
00:05
Title
00:39
Cell Culture for Immunofluorescence Microscopy
01:32
Preparation of Transfection Complex and Transfection of Cells
03:08
Live Cell Imaging and Indirect Immunofluorescence
05:40
Results: Identifying Kinesin Cargo via Fluorescence Microscopy
06:50
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,提出一个协议,以确定驱动蛋白-1的货物。该驱动蛋白1重链(KIF5B)的无电机突变体聚集在细胞质和诱导其货物的聚集。两个聚集体在荧光显微镜下检测。类似的策略可以用于识别其他马达蛋白的货物。
Tags
Kinesin-1
Fluorescence Microscopy
Cargos
Molecular Motors
Myosins
HeLa Cells
Indirect Immunofluorescence
Transfection
TdTomato
KIF5B
Live Cell Imaging
Hoechst 33342
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