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Developmental Biology
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ヒト胚性幹細胞から生成された内胚葉細胞の精製のための迅速かつ効率的な方法
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JoVE Journal
Developmental Biology
A Quick and Efficient Method for the Purification of Endoderm Cells Generated from Human Embryonic Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
ヒト胚性幹細胞から生成された内胚葉細胞の精製のための迅速かつ効率的な方法
DOI:
10.3791/53655-v
•
08:34 min
•
March 03, 2016
•
Claudia Davenport
,
Ulf Diekmann
,
Ortwin Naujok
1
Institute of Clinical Biochemistry
,
Hannover Medical School
Chapters
00:05
Title
00:34
Human ES Cell Differentiation toward the Definitive Endoderm
02:57
CXCR4+ Definitive Endoderm (DE) Cell Immunostaining for Flow Cytometric Analysis
04:55
CXCR4+ Cell Magnetic Bead Isolation
06:34
Results: Representative Changes in Gene/Protein Expression after Human ESC into DE Differentiation
07:59
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここでは、下流のアプリケーション、さらに分化の改善のための胚体内胚葉に向けてコミットしている分化したヒト胚性幹細胞を精製するための方法を説明します。
Tags
Endoderm Cells
Human Embryonic Stem Cells
Purification
Differentiation
Cell Culture
Basement Membrane Matrix
ROCK Inhibitor
Primitive Streak Induction
Endoderm Induction
Single-cell Suspension
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