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Hücresel Mitophagy için bir Marker olarak EYFP-Parkin Toplama Değerlendirme Time-Lapse video Mikroskopi
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Time-Lapse Video Microscopy for Assessment of EYFP-Parkin Aggregation as a Marker for Cellular Mitophagy
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Hücresel Mitophagy için bir Marker olarak EYFP-Parkin Toplama Değerlendirme Time-Lapse video Mikroskopi

DOI:
10.3791/53657-v

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09:29 min

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May 04, 2016

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, , ,
1Department of Cancer Biology,Thomas Jefferson University, SKCC, 2Department of Medical Oncology,Thomas Jefferson University, SKCC, 3Kazan Federal University

Chapters

  • 00:05Title
  • 00:49Electroporation of Fibroblasts with EYFP-Parkin and pDsRed2-Mito Expression Vectors
  • 03:44Time-lapse Video Microscopy
  • 07:20Results: EYFP-Parkin Aggregates in Mitochondrial Membranes During Mitophagy
  • 08:55Conclusion

Summary

Automatic Translation

Automatic Translation

Herein, we describe in detail a time-lapse video microscopy approach to measuring the temporal recruitment of EYFP-Parkin during the selective removal of damaged mitochondria. This dynamic process of EYFP-Parkin-dependent removal of damaged mitochondria can be used as an indicator of cellular health under different experimental conditions.

Tags

Time-Lapse Video MicroscopyEYFP-Parkin AggregationCellular MitophagyMolecular MetabolismLabeled ProteinsMitochondrial Life CyclePrimary Cell CulturesEmbryology StudiesPredicting PriorityImmortalized Mouse Embryonic FibroblastsDMEM MediumTrypsin EDTACell Suspension

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