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分离和胶质瘤浸润外周血单个核细胞的流式细胞仪分析
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Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
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分离和胶质瘤浸润外周血单个核细胞的流式细胞仪分析

DOI:
10.3791/53676-v

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12:52 min

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November 28, 2015

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, ,
1Department of Neurosurgery,University of Michigan, 2Department of Cell and Developmental Biology,University of Michigan

Chapters

  • 00:05Title
  • 00:46Stereotactic Engraftment of Glioma Cells into Mouse Striatum
  • 02:52Mouse Transcardial Perfusion and Brain Harvesting
  • 04:31Isolation of Glioma-infiltrating PBMCs
  • 09:40Results: Flow Cytometric Gating Strategy Used to Identify Glioma-infiltrating Gr-1+/ CD11b+ Myeloid Cells and NK.1 + Cells
  • 11:27Conclusion

Summary

Automatic Translation

Automatic Translation

这里介绍的是胶质瘤浸润外周血单核细胞的分离和流式细胞术分析能够产生在进入早期脑肿瘤微环境的免疫细胞的数目和激活状态依赖于时间的定量数据一个直接的方法。

Tags

GliomaPeripheral Blood Mononuclear CellsFlow CytometryNatural Killer CellsMyeloid CellsGalectin-1Tumor MicroenvironmentImmune InfiltrationBrain Tumor

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