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体外肺转移及微环境的实时成像
JoVE Journal
Medicine
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JoVE Journal Medicine
Ex vivo Live Imaging of Lung Metastasis and Their Microenvironment
DOI:

11:19 min

February 03, 2016

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Chapters

  • 00:05Title
  • 01:03Lung Preparation for Ex vivo Live Imaging
  • 05:16Image Acquisition and Analysis
  • 08:36Results: Ex vivo Imaging of Lung Metastasis and Their Microenvironment
  • 10:20Conclusion

Summary

Automatic Translation

我们描述用于离体肺转移中的肿瘤细胞-基质相互作用的实时成像的相对简单的方法,利用荧光报告小鼠。使用纺丝盘共焦显微镜,该技术使活细胞的可视化的至少4小时,并可以适用于研究其它炎性肺部疾病。

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