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Ex vivo Levende Imaging af lungemetastase og deres mikromiljø
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JoVE Journal Medicine
Ex vivo Live Imaging of Lung Metastasis and Their Microenvironment

Ex vivo Levende Imaging af lungemetastase og deres mikromiljø

DOI:
10.3791/53741-v

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11:19 min

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February 03, 2016

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, , ,
1Department of Anatomy,University of California, 2Hubrecht Institute-Royal Dutch Academy of Science and University Medical Center Utrecht, 3Department of Cell and Developmental Biology,Sackler Faculty of Medicine

Chapters

  • 00:05Title
  • 01:03Lung Preparation for Ex vivo Live Imaging
  • 05:16Image Acquisition and Analysis
  • 08:36Results: Ex vivo Imaging of Lung Metastasis and Their Microenvironment
  • 10:20Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver en forholdsvis enkel fremgangsmåde til ex vivo billeddannelse af tumor celle-stroma interaktioner inden lunge metastase, udnytte fluorescerende reportere i mus. Brug af spinning-disk konfokal mikroskopi, denne teknik muliggør visualisering af levende celler i mindst 4 timer, og kan tilpasses til at studere andre inflammatoriske lungesygdomme.

Tags

Ex Vivo Live ImagingLung MetastasisTumor Cell Stroma InteractionsMetastasis EstablishmentCell TypesMicroenvironmentTargeted TherapeuticsLung ImagingCell DeathMouseTracheaAgarose

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