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Chapters
- 00:05Title
- 01:03Lung Preparation for Ex vivo Live Imaging
- 05:16Image Acquisition and Analysis
- 08:36Results: Ex vivo Imaging of Lung Metastasis and Their Microenvironment
- 10:20Conclusion
We describe a relatively simple method for ex vivo live imaging of the tumor cell-stroma interactions within lung metastasis, utilizing fluorescent reporters in mice. Using spinning-disk confocal microscopy, this technique enables visualization of live cells for at least 4 hr and could be adapted to study other inflammatory lung conditions.
Tags
Ex Vivo Live ImagingLung MetastasisTumor Cell-stroma InteractionsMicroenvironmentCancer MetastasisCell TypesVisualization Of Live CellsTargeted TherapeuticsLung Section ImagingCell DeathPolystyrene Foam LidSterilize The AnimalTransverse Epigastric IncisionPeritoneumDescending AortaVacuum ReleaseRib Cage Excision
