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Neuroscience
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用优化的
n-
甲基 d-葡乙胺保护恢复法制备急性脑切片
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Preparation of Acute Brain Slices Using an Optimized
N
-Methyl-D-glucamine Protective Recovery Method
Please note that all translations are automatically generated.
Click here for the English version.
用优化的
n-
甲基 d-葡乙胺保护恢复法制备急性脑切片
DOI:
10.3791/53825-v
•
10:53 min
•
February 26, 2018
•
Jonathan T. Ting*
1
,
Brian R. Lee*
1
,
Peter Chong
,
Gilberto Soler-Llavina
,
Charles Cobbs
,
Christof Koch
,
Hongkui Zeng
,
Ed Lein
1
Cell Types Program
,
Allen Institute for Brain Science
,
2
The Ben and Catherine Ivy Center for Advanced Brain Tumor Treatment
,
Swedish Neuroscience Institute
Chapters
00:04
Title
01:05
Setup of the Slicing Station
03:15
Brain Dissection and Slicing
06:32
Optimized NMDG Protective Recovery Procedure
08:05
Results: Improved Neuronal Preservation and Reliability of Gigaohm Seal Formation with the Optimized NMDG Protective Recovery Method
09:38
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议演示了一种优化的
N
-甲基 D-葡乙胺 (NMDG) 脑切片制备保护恢复方法的实现。一个单一的媒体配方是用来可靠地获得健康的脑切片从动物的任何年龄和不同的实验应用。
Tags
Brain Slices
NMDG Protective Recovery Method
Patch Clamp Electrophysiology
Neuronal Preservation
Adult Mouse Brain
NMDG HEPES ACSF
Brain Slice Recovery Chamber
HEPES ACSF
Agarose Embedding
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