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Assessing Primary Neurogenesis in Xenopus Embryos Using Immunostaining
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Neuroscience
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JoVE Journal Neuroscience
Assessing Primary Neurogenesis in Xenopus Embryos Using Immunostaining

Assessing Primary Neurogenesis in Xenopus Embryos Using Immunostaining

DOI:
10.3791/53949-v

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06:47 min

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April 12, 2016

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, , ,
1The Healing Foundation Centre, Faculty of Life Sciences,University of Manchester, 2Department of Cell and Molecular Biology, Feinberg School of Medicine,Northwestern University, 3Department of Craniofacial Development and Stem Cell Biology, Dental Institute,King’s College London

Chapters

  • 00:05Title
  • 00:51Mouting Xenopus Embryos
  • 02:05Cryosection of Mounted Xenopus Embryo
  • 05:04Results: Cross-section of X. leavis Tadpoles and Staining in the Neural Tissue
  • 05:47Conclusion

Summary

Automatic Translation

Automatic Translation

This article presents a convenient and rapid method for visualizing different neuronal cell populations in the central nervous system of Xenopus embryos using immunofluorescent staining on sections.

Tags

Keywords: Primary NeurogenesisXenopus EmbryosImmunostainingNeural Stem-cell Progenitor PoolDifferentiated Primary Neural PoolCryosectioningMicrotomeCentral Nervous SystemNeuronal Differentiation

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