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该<em>离体</em>文化与模式识别小鼠肠道组织体受体刺激
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Immunology and Infection
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JoVE Journal Immunology and Infection
The Ex Vivo Culture and Pattern Recognition Receptor Stimulation of Mouse Intestinal Organoids
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该<em>离体</em>文化与模式识别小鼠肠道组织体受体刺激

DOI:
10.3791/54033-v

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09:09 min

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May 18, 2016

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, , , ,
1Department of Biomedical Sciences and Pathobiology,Virginia Maryland College of Veterinary Medicine, Virginia Tech, 2Department of Biomedical Engineering,Cornell University, 3School of Electrical and Computer Engineering,Cornell University, 4Department of Biomedical Engineering,Duke University

Chapters

  • 00:05Title
  • 00:46Mus musculus Small Intestinal Crypt Harvest
  • 03:57Organoid Plating for Pathogen Associated Molecular Patters (PAMP) and L. monocytogenes Challenge
  • 06:58Results: Representative Mouse Intestinal Organoid Culture and Stimulation
  • 08:29Conclusion

Summary

Automatic Translation

Automatic Translation

这里，收获，维护和治疗与病原体相关的分子模式（PAMP），并李斯特菌小鼠小肠组织体的协议被描述，以及强调基因表达和蛋白质正确规范化技术。

Tags

Mouse Intestinal OrganoidsEx Vivo CulturePattern Recognition Receptor StimulationInnate ImmunityMucosal ImmunologyHost-pathogen InteractionsIntestinal Epithelial CellsELISAIntestinal CryptsNormalizationCell NumberPBSEDTADMEM/F12Cell FractionationCrypt Visualization

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