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在C2C12肌细胞造型强直性肌营养不良1
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JoVE Journal Medicine
Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells
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在C2C12肌细胞造型强直性肌营养不良1

DOI:
10.3791/54078-v

•

09:39 min

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July 29, 2016

•

, , , , ,
1Department of Biology and Biochemistry,University of Houston, 2Department of Economics,University of Houston, 3Department of Cardiology,The First Affiliated Hospital of Nanchang University

Chapters

  • 00:05Title
  • 00:40C2C12 Transfection
  • 02:06C2C12 Retroviral Transduction
  • 04:44C2C12 Lentiviral Transduction
  • 06:46Results: The Effects of Celf1 in Myoblast Differentiation
  • 08:53Conclusion

Summary

Automatic Translation

Automatic Translation

在这个协议中，我们提出在建立强直性肌营养不良1成肌细胞模型，包括优化C2C12细胞维持，基因转染/转导，和肌细胞分化的步骤。

Tags

C2C12 MyoblastsMyotonic DystrophyM1 Cell ModelGene ManipulationTransfectionTransductionRetrovirusPackaging CellsSelection AntibioticCell CultureCell MorphologyCytotoxicity

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