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在C2C12肌细胞造型强直性肌营养不良1
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Medicine
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JoVE Journal
Medicine
Modeling Myotonic Dystrophy 1 in C2C12 Myoblast Cells
Please note that all translations are automatically generated.
Click here for the English version.
在C2C12肌细胞造型强直性肌营养不良1
DOI:
10.3791/54078-v
•
09:39 min
•
July 29, 2016
•
Rui Liang*
1
,
Wei Dong*
1,3
,
Xiaopeng Shen
,
Xiaoping Peng
3
,
Angie G. Aceves
2
,
Yu Liu
1
Department of Biology and Biochemistry
,
University of Houston
,
2
Department of Economics
,
University of Houston
,
3
Department of Cardiology
,
The First Affiliated Hospital of Nanchang University
Chapters
00:05
Title
00:40
C2C12 Transfection
02:06
C2C12 Retroviral Transduction
04:44
C2C12 Lentiviral Transduction
06:46
Results: The Effects of Celf1 in Myoblast Differentiation
08:53
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这个协议中,我们提出在建立强直性肌营养不良1成肌细胞模型,包括优化C2C12细胞维持,基因转染/转导,和肌细胞分化的步骤。
Tags
C2C12 Myoblasts
Myotonic Dystrophy
M1 Cell Model
Gene Manipulation
Transfection
Transduction
Retrovirus
Packaging Cells
Selection Antibiotic
Cell Culture
Cell Morphology
Cytotoxicity
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