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Methods for Acute and Subacute Murine Hindlimb Ischemia

Methods for Acute and Subacute Murine Hindlimb Ischemia

Article DOI: 10.3791/54166-v 07:57 min June 21st, 2016
June 21st, 2016



Surgical induction of hindlimb ischemia in the mouse is useful to examine angiogenesis, however this is compromised in certain inbred mouse strains that display marked ischemia-induced tissue necrosis. Methods are described to induce subacute limb ischemia using ameroid constrictors to circumvent this problem through the induction of gradual arterial occlusion.


Acute Hindlimb Ischemia Subacute Hindlimb Ischemia Peripheral Artery Disease Mouse Femoral Artery Sutures Ameroid Constrictors Animal Models Limb Ischemia Control Sedation Hair Removal Surgical Procedure Dissection Microscope Surgical Tape Povidone
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