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Measuring In Vitro ATPase Activity for Enzymatic Characterization
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JoVE Journal Biology
Measuring In Vitro ATPase Activity for Enzymatic Characterization

Measuring In Vitro ATPase Activity for Enzymatic Characterization

DOI:
10.3791/54305-v

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07:38 min

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August 23, 2016

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, ,
1Department of Microbiology and Immunology,University of Michigan

Chapters

  • 00:05Title
  • 00:36Perform ATP Hydrolysis Reaction with Purified Protein
  • 03:09Incubate Samples Containing Free Pi with Detection Reagent
  • 05:44Results: Quantitation of In Vitro ATPase Activity
  • 06:59Conclusion

Summary

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We describe a basic protocol for quantitating in vitro ATPase activity. This protocol can be optimized based on the level of activity and requirements for a given purified ATPase.

Tags

ATPase ActivityIn Vitro CharacterizationATP HydrolysisProtein FunctionEnzyme AssayMagnesium ChlorideBufferDilutionSample CollectionDry Ice Ethanol BathTime CourseSample Freezing

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