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Developmental Biology
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在微孔聚集尺寸优化人类多能干细胞的基于悬浮心脏分化
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Aggregate Size Optimization in Microwells for Suspension-based Cardiac Differentiation of Human Pluripotent Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
在微孔聚集尺寸优化人类多能干细胞的基于悬浮心脏分化
DOI:
10.3791/54308-v
•
06:28 min
•
September 25, 2016
•
Celine L. Bauwens*
1
,
Derek Toms*
2
,
Mark Ungrin
1
Institute of Biomaterials and Biomedical Engineering
,
University of Toronto
,
2
Department of Comparative Biology and Experimental Medicine
,
University of Calgary
Chapters
00:05
Title
00:54
Plating hPSC Aggregates in the Microwell Plate
02:00
Cardiac Induction Stage 1
03:06
Cardiac Induction Stage 2
04:16
Cardiac Induction Stage 3
05:06
Results: Characteristics of Differentiated hPSCs
05:44
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
常规的方法以引发人多能的悬浮聚合基于心脏分化干细胞(hPSCs)的困扰与培养异质相对于聚集体的尺寸和形状。在这里,我们描述了采用微孔产生大小控制HPSC心脏推进的条件下培养聚集心脏分化一个可靠的方法。
Tags
Aggregate Size Optimization
Microwells
Suspension-based Cardiac Differentiation
Human Pluripotent Stem Cells
Centrifugation
Cell Suspension
Microwell Plate
Hypoxic Incubation
Aggregate Harvesting
Induction Medium
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