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Microfluídico troca de tampão para Micro / nanopartículas celular Engenharia sem interferências
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
DOI:

10:27 min

July 10, 2016

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Chapters

  • 00:05Title
  • 01:16Nanoparticle Labeling of Mesenchymal Stem Cells and Monocytes
  • 03:29Microfluidic Device Fabrication
  • 06:17Operating the Microfluidic Device
  • 09:04Results: Dean Flow Fractionation of Nano-particle Labeled Monocytes (THP-1)
  • 09:59Conclusion

Summary

Automatic Translation

Este protocolo descreve o uso de uma estratégia de troca de tampão à base de microfluidos inercial para purificar as células manipuladas micro / nanopartículas com depleção eficaz de partículas não ligadas.

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