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荧光各向异性作为一种工具来研究蛋白质相互作用
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Fluorescence Anisotropy as a Tool to Study Protein-protein Interactions
DOI:

10:44 min

October 21, 2016

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Chapters

  • 00:05Title
  • 00:36SBDS-FlAsH Tag Protein Expression and Purification
  • 04:10Elongation Factor Like-1 (EFL1) Protein Expression and Purification
  • 07:07Labeling of SBDS-FlAsH with the FlAsH Fluorescent Dye 4′,5′-Bis(1,3,2 dithioarsolan-2-yl) Fluorescein and Fluorescence Anisotropy Experiments
  • 08:50Results: Evaluation of Protein-protein Interactions Using Fluorescence Anisotropy
  • 09:55Conclusion

Summary

Automatic Translation

蛋白质相互作用是在一个细胞的功能的心脏。量热和光谱技术通常用于表征它们。这里我们介绍荧光各向异性作为一种工具来研究在Shwachman-Diamond综合征(SBDS)突变蛋白质和延伸因子样1 GTP酶(EFL1)之间的相互作用。

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