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Biochemistry

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JoVE Journal Biochemistry
Fluorescens anisotropi som et verktøy for å studere protein-protein interaksjoner
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Fluorescens anisotropi som et verktøy for å studere protein-protein interaksjoner

Article DOI: 10.3791/54640-v • 10:44 min • October 21st, 2016
October 21st, 2016 •
1, 2, 1
1Departamento de Química de Biomacromoléculas, Instituto de Química, Universidad Nacional Autónoma de México, 2Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México

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Protein interaksjoner er i hjertet av en celle funksjon. Kalorimetriske og spektroskopiske teknikker blir ofte brukt for å karakterisere dem. Her beskriver vi fluorescens anisotropi som et verktøy for å studere samspillet mellom protein mutert i Shwachman-Diamond syndrom (SBDS) og Forlengelse faktor-lignende en GTPase (EFL1).

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Biokjemi fluorescens anisotropi fluorescens Forlengelse faktor-lignende en Shwachman-Diamond syndrom protein Protein-protein interaksjon fluorophore
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