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A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma
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JoVE Journal Cancer Research
A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma

A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma

DOI:
10.3791/55043-v

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08:59 min

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January 05, 2017

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, , , ,
1Department of Otolaryngology,University of Pittsburgh Medical Center, 2University of Pittsburgh Cancer Institute,Hillman Cancer Center

Chapters

  • 00:05Title
  • 00:46Murine Dorsal Root Ganglion (DRG) Dissection
  • 03:39Semisolid Matrix Droplets Preparation, DRG Insertion, and Head and Neck Cancer Cell Plating
  • 06:44Results: Representative Neurite Outgrowth Analyses
  • 08:07Conclusion

Summary

Automatic Translation

Automatic Translation

Perineural invasion (PNI) is a common feature of head and neck squamous cell carcinoma (HNSCC), conferring lower survival rates. Its mechanisms are poorly understood. Utilizing neurites generated from murine dorsal root ganglia confined to a semisolid matrix, the pathways involved in the PNI of HNSCC cell lines can be investigated.

Tags

Keywords: Perineural InvasionHead And Neck Squamous Cell CarcinomaDorsal Root GanglionDRG DissectionMouse ModelSpinal CordPeripheral NerveMicroscopic Procedures

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