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一个单一的鼠标骨骼肌的相邻区Cryosectioning的基因表达和组织学分析
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Biology
Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses
Please note that all translations are automatically generated.
Click here for the English version.
一个单一的鼠标骨骼肌的相邻区Cryosectioning的基因表达和组织学分析
DOI:
10.3791/55058-v
•
08:17 min
•
December 12, 2016
•
Aaron M. Beedle
1
Department of Pharmaceutical and Biomedical Sciences
,
University of Georgia
Chapters
00:05
Title
00:50
Cryopreservation
02:04
Collecting Cryosections for RNA Extraction and Histology
04:20
RNA Isolation from Pooled Cryosections
06:17
Results: RNA Extraction and Histology of Cardiotoxin Injured Muscle
07:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
连续的低温部分被收集,以便利用邻近地区从一个单一的小鼠骨骼肌基因表达测量组织的应用程序和RNA的富集。 30毫克汇集冷冻切片和测量的应用程序之间直接比较 - 高品质的RNA是从20获得。
Tags
Cryosectioning
Contiguous Regions
Single Mouse Skeletal Muscle
Gene Expression
Histological Analyses
Regenerative Effects
Muscular Injury
Viral Gene Delivery
Intramuscular Injection
Histological Data
Molecular Data
Embedding Resin
2-Methylbutane
Liquid Nitrogen
Cryostat
RNA Extraction
Tibialis Anterior
Article
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