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JoVE Journal
Immunology and Infection

This content is Open Access.

成像中性粒细胞吞噬体和细胞质使用比例测量pH指示剂
 

成像中性粒细胞吞噬体和细胞质使用比例测量pH指示剂

Article doi: 10.3791/55107
April 5th, 2017

Summary April 5th, 2017

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这手稿描述了一种简单的方法来测量吞噬体的pH值和面积以及使用比率量度指示器seminaphthorhodafluor(SNARF)-1,或S-1人和小鼠嗜中性粒细胞的胞质pH值。这是使用活细胞共聚焦荧光显微镜和图像分析来实现的。

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