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Immunology and Infection
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通过“Acufection”和它的皮肤研究中的应用的经济型DNA递送系统的开发
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Immunology and Infection
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Immunology and Infection
Development of an Economical DNA Delivery System by “Acufection” and its Application to Skin Research
Please note that all translations are automatically generated.
Click here for the English version.
通过“Acufection”和它的皮肤研究中的应用的经济型DNA递送系统的开发
DOI:
10.3791/55206-v
•
09:42 min
•
April 19, 2017
•
Yu-Jei Lin
,
Tsung-Lin Lee
,
Chia-Chi Ku
1
Graduate Institute of Immunology
,
National Taiwan University College of Medicine
Chapters
00:05
Title
00:40
Large Scale Preparation of Endotoxin-free Plasmid DNA
01:36
Acufection Procedure and Post-operative Care
03:17
Imiquimod Treatment of IL-15ΔE7-acufected Skin
04:06
Mouse Skin Homogenation
05:20
Immunohistochemical Staining of the Skin Section
07:23
Results: Acufection Enables an Efficient Gene Trasfer While Inducing Minimal Skin Damage
08:37
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这个协议是用于在小鼠皮肤中表达裸质粒DNA具有成本效益的选择。该协议的总体目标是提供免疫相关基因植入皮肤组织划定的皮肤炎症特定基因的功能作用。
Tags
DNA Delivery
Acufection
Skin Research
Cutaneous Inflammation
Psoriasis
Plasmid DNA
Bacterial Culture
LB Medium
Endotoxin-free Plasmid Preparation
PBS
Depilatory Cream
Ethanol Disinfectant
Acupuncture Apparatus
Plasmid DNA Expression
Anesthesia
Heating Pad
Mouse Monitoring
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