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Developmental Biology
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小儿食管上皮细胞重编程的条件在组织工程和疾病使用调查
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation
Please note that all translations are automatically generated.
Click here for the English version.
小儿食管上皮细胞重编程的条件在组织工程和疾病使用调查
DOI:
10.3791/55243-v
•
10:15 min
•
March 22, 2017
•
Todd J. Jensen
,
Christopher Foster
,
Wael Sayej
,
Christine M. Finck
1
Department of Pediatrics
,
UConn Health
,
2
Department of Gastroenterology
,
Connecticut Children’s Medical Center
,
3
Department of Surgery
,
Connecticut Children’s Medical Center
Chapters
00:05
Title
01:21
3T3 Feeder Cell Culture and Irradiation
02:31
Pediatric Esophageal Biospy Acquisition and Processing
05:00
Cell Culture Expansion, Freezing, and Storage
07:17
Results: Representative Human Esophageal Cell Characterization
08:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
利用条件重新编程人类小儿食管上皮细胞的扩张提供了可用于工程食管构造为自体移植来治疗缺损或损伤,并作为治疗性筛选测定的储存器单元的特定病人群调查。
Tags
Conditional Reprogramming
Esophageal Epithelial Cells
Disease Investigation
Eosinophilic Esophagitis
Esophageal Atresia
3D Scaffold
Patient-specific
Endoscopic Biopsy
Feeder Cells
3T3 Cells
Irradiation
Conditional Programming Medium
Serum-free Keratinocyte Medium
Primocin
Formalin
Cryomold
Article
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