Journal
/
Immunology and Infection
/
小鼠淋巴细胞通过标签<sup> 64</sup>的Cu-抗体受体靶向用于<em>体内</em>由PET / CT细胞运输
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT
Please note that all translations are automatically generated. Click here for the English version.

小鼠淋巴细胞通过标签<sup> 64</sup>的Cu-抗体受体靶向用于<em>体内</em>由PET / CT细胞运输

DOI:
10.3791/55270-v

•

11:34 min

•

April 29, 2017

•

, , , , , ,
1Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy,Eberhard Karls University Tübingen, 2Department of Dermatology,Eberhard Karls University Tübingen

Chapters

  • 00:05Title
  • 01:13Chicken Ovalbumin-specific TH1 (cOVA-TH1) Cell isolation and Antigen Presenting Cell Co-culture
  • 04:42cOVA-TH1 Cell Radiolabeling and Uptake and Efflux Analysis
  • 06:44Positron Emission Tomograph–Computed Tomography (PET/CT) In Vivo Imaging
  • 08:26Results: Representative cOVA-TH1 Cell Tracking In Vitro and In Vivo
  • 10:32Conclusion

Summary

Automatic Translation

Automatic Translation

以下64的Cu改性的单克隆抗体结合到转基因小鼠的T细胞受体的制备中，T细胞在体内的放射性标记的，用于存活力，功能，标记稳定性和细胞凋亡分析，并过继转移到小鼠的气道迟发型超敏反应用于非侵入性成像通过正电子发射断层成像/计算机断层扫描（PET / CT）反应。

Tags

Murine Lymphocytes64Cu-antibody ReceptorPET/CTCell TraffickingInflammationCancerCell-based TherapiesRegenerative MedicineCancer ImmunotherapiesCell MigrationCell LabelingChelator ConjugationRadiolabelingLymph NodesSpleenCD4-positive CellsMagnetic Cell Separation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

小鼠骨髓来源的树突状细胞与跟踪研究的Qdot纳米晶的生成及标签

诱导小鼠肠道炎症由效应CD4的过继转移<sup> +</sup> CD45RB<sup>高</sup> T细胞免疫缺陷小鼠

从表型分析和人体皮肤淋巴细胞分离<em>离体</em>细胞培养

股骨窗口商会模型<em>在体内</em在小鼠骨髓>手机跟踪

检测<em>布氏锥虫</em>变体表面糖蛋白通过磁性细胞分选和流式细胞仪开关

非侵入性 体内生物发光系统检测小鼠宿主肠出血性大肠杆菌的定植

人单核细胞趋化体外和小鼠淋巴细胞贩运体内

分析18葡萄糖 PET/CT 成像作为研究结核分枝杆菌感染和治疗非人类灵长类动物的工具

荧光寿命成像显微镜观察细胞内圈套的贩运

外迁移系统中淋巴细胞迁移的评估

Read Article