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Journal
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Immunology and Infection
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使用成像流式细胞仪T细胞同种异体反应性测定
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal
Immunology and Infection
Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
使用成像流式细胞仪T细胞同种异体反应性测定
DOI:
10.3791/55283-v
•
09:04 min
•
April 19, 2017
•
Stephen C. Juvet
,
Sajad Moshkelgosha
,
Sharon Sanderson
,
Joanna Hester
,
Kathryn J. Wood
,
Andrew Bushell
1
Division of Respirology, Departments of Medicine and Immunology, Toronto Lung Transplant Program, Multiorgan Transplant Program, Toronto General Research Institute
,
University of Toronto and University Health Network
,
2
Latner Thoracic Surgery Laboratories, Toronto General Research Institute
,
University Health Network
,
3
National Institutes of Health Research, Oxford Biomedical Research Centre, Translational Immunology Laboratory, NDORMS, Kennedy Institute of Rheumatology
,
University of Oxford
,
4
Transplantation Research Immunology Group, Nuffield Department of Surgical Sciences, John Radcliffe Hospital
,
University of Oxford
Chapters
00:05
Title
00:45
T Cell and Antigen-presenting Cell (APC) Co-incubation
02:52
Data Acquisition
04:25
Data Analysis
07:39
Results: Representative T Cell: APC Synapse Formation and Analyses
08:37
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文描述了用于在使用成像流式细胞仪的T细胞的混合群测量同种异体反应性的方法。
Tags
T Cell Alloreactivity
Imaging Flow Cytometry
Transplant Immunology
Dendritic Cells
T Cells
Cell-cell Interactions
Fluorescent Microscopy
Flow Cytometry
Cell Culture
Fixation
Staining
Perm/wash Buffer
Nuclear Dye
Brightfield Imaging
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