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Journal
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Developmental Biology
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隔离并通过流式细胞仪分析胰腺间充质细胞
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Isolating and Analyzing Cells of the Pancreas Mesenchyme by Flow Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
隔离并通过流式细胞仪分析胰腺间充质细胞
DOI:
10.3791/55344-v
•
05:38 min
•
January 28, 2017
•
Alona Epshtein
,
Lina Sakhneny
,
Limor Landsman
1
Cell and Developmental Biology
,
Sackler Faculty of Medicine, Tel Aviv University
Chapters
00:05
Title
00:39
Pancreas Digestion and Fluorescence-activated Cell Sorting (FACS)
03:54
Results: Representative Isolated Pancreatic Cell Analyses
05:13
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里,我们描述了一种用于细胞在胰腺微环境从胚胎,新生儿和成年小鼠组织的隔离,着眼于间充质细胞的分离。此方法允许细胞基因表达和蛋白质分泌的分析以阐明调节胰腺发育,功能,和肿瘤发生的外在信号。
Tags
Pancreas
Mesenchyme
Flow Cytometry
Cell Isolation
Gene Expression
Surface Marker
Pancreas Development
Epithelial Development
Cell Sorting
Digestive Buffer
HBSS
Centrifugation
Cell Strainer
Sorting Buffer
Viability Dye
FACS
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