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器官海马切片培养模型研究肿瘤细胞的神经保护和侵袭性
JoVE Journal
Neuroscience
Author Produced
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JoVE Journal Neuroscience
Organotypic Hippocampal Slice Cultures As a Model to Study Neuroprotection and Invasiveness of Tumor Cells
DOI:

07:48 min

August 27, 2017

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Chapters

  • 00:00Title
  • 00:08Agar Preparation
  • 00:54Brain Fixation
  • 02:29Slicing Procedure
  • 03:26Hippocampus Removal
  • 04:05Culturing Method
  • 05:18Representative Results
  • 07:19Conclusion

Summary

Automatic Translation

器官海马切片培养 (OHSC) 代表一个体外模型, 它能很好地模拟体内的情况。在这里, 我们描述一个 vibratome-based 改进的切片协议, 以获得高质量的切片, 用于评估新物质的神经保护潜能或肿瘤细胞的生物学行为。

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