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단일 셀 TCR 고립과 Retroviral 벡터 생체 외에서 의 생성과 Vivo에서 표현의 인간의 TCRs 간소화
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1Department of Pediatrics, Section of Diabetes and Endocrinology, McNair Medical Institute,Baylor College of Medicine, Texas Children’s Hospital, 2Benaroya Research Institute at Virginia Mason, 3Center for Human Immunobiology,Baylor College of Medicine, Texas Children’s Hospital, 4Department of Pediatrics, Section of Diabetes and Endocrinology,Baylor College of Medicine, Texas Children’s Hospital
Chapters
- 00:00Title
- 01:42Single cell sort and reverse transcription reaction
- 03:37Multiplex-nested PCR
- 08:56Subcloning TCR alpha and beta chains
- 09:56Verification of TCR expression
- 10:46Conclusion
