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简化的单细胞 TCR 分离和逆转录病毒载体的生成, 用于体外体内表达人类 TCRs
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
DOI:

11:21 min

September 10, 2017

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Chapters

  • 00:00Title
  • 01:42Single cell sort and reverse transcription reaction
  • 03:37Multiplex-nested PCR
  • 08:56Subcloning TCR alpha and beta chains
  • 09:56Verification of TCR expression
  • 10:46Conclusion

Summary

Automatic Translation

当前的协议结合了单细胞配对人类 TCR α和β链测序与与体外体内TCR 表达相兼容的反转录病毒载体的生成。

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