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Journal / Immunology and Infection / 简化的单细胞 TCR 分离和逆转录病毒载体的生成, 用于体外和体内表达人类…
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Immunology and Infection
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JoVE Journal Immunology and Infection
简化的单细胞 TCR 分离和逆转录病毒载体的生成, 用于体外和体内表达人类 TCRs
Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
Journal (Immunology and Infection)
Isolation, Expansion, and Adipogenic Induction of CD34+CD31+ Endothelial Cells from Human Omental and Subcutaneous Adipose Tissue
Human Placental and Decidual Organ Cultures to Study Infections at the Maternal-fetal Interface
Journal (Immunology and Infection)
Human Placental and Decidual Organ Cultures to Study Infections at the Maternal-fetal Interface
 
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简化的单细胞 TCR 分离和逆转录病毒载体的生成, 用于体外和体内表达人类 TCRs

Article DOI: 10.3791/55379-v • 11:21 min • September 10th, 2017
September 10th, 2017 •
1, 2, 1, 1, 3, 2, 4, 1, 1
1Department of Pediatrics, Section of Diabetes and Endocrinology, McNair Medical Institute, Baylor College of Medicine, Texas Children's Hospital, 2Benaroya Research Institute at Virginia Mason, 3Center for Human Immunobiology, Baylor College of Medicine, Texas Children's Hospital, 4Department of Pediatrics, Section of Diabetes and Endocrinology, Baylor College of Medicine, Texas Children's Hospital

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当前的协议结合了单细胞配对人类 TCR α和β链测序与与体外和体内TCR 表达相兼容的反转录病毒载体的生成。

Tags

免疫学 问题 127 t 细胞 t 细胞受体 TCR PCR 测序 逆转录病毒 单细胞 人,在体内,体外 TCR 表达
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