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Aerodinâmico única célula TCR isolamento e geração de vetores retrovirais para In Vitro e In Vivo de expressão de TCRs humanas
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
DOI:

11:21 min

September 10, 2017

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Chapters

  • 00:00Title
  • 01:42Single cell sort and reverse transcription reaction
  • 03:37Multiplex-nested PCR
  • 08:56Subcloning TCR alpha and beta chains
  • 09:56Verification of TCR expression
  • 10:46Conclusion

Summary

Automatic Translation

O atual combina de protocolo único celular emparelhado humana TCR alfa e beta cadeia sequenciamento com simplificada geração de vetores retrovirais compatíveis com expressão de TCR in vitro e in vivo .

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