JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Developmental Biology
/
一种在96孔板表演细胞迁移/伤口愈合设备
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Developmental Biology
A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate
Please note that all translations are automatically generated.
Click here for the English version.
一种在96孔板表演细胞迁移/伤口愈合设备
DOI:
10.3791/55411-v
•
05:46 min
•
March 07, 2017
•
Po Ying Poon
,
Patrick Ying Kit Yue
,
Ricky Ngok Shun Wong
1
Department of Biology
,
Hong Kong Baptist University
Chapters
00:05
Title
00:59
Introducing the Wounder Parts
01:38
Setting the Pin Holder Width
02:05
Adjusting the Guiding Bars
02:49
Calibrating the Pins
03:23
Scratching the Cell Monolayer
04:44
Results: Uniform Wound Width with Device
05:13
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这里,我们提出了一个协议,以在96孔细胞培养板进行半高通量细胞迁移测定。该协议是建立在一个细胞单层一致划伤伤口快速,简便,经济的方法。
Tags
Cell Migration
Wound Healing
96-well Plate
In Vitro Assay
Cell Migration Quantification
Semi-high Throughput
Adjustable Wounding Pins
Guiding Bar
Pipet Tips
Hex Screw
Hex Socket Head Caps
Adjusting Rings
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
了解早期器官使用简体<em>原位</em>杂交协议<em>爪</em
造血干细胞的自动定量 - 在脱钙骨的组织学图像间质细胞的相互作用
可扩展的96孔板基于IPSC的文化和生产用机器人液体处理系统
建立临床相关<em>离体</em>调查上皮创伤修复的微环境本地人模拟白内障手术模型
生成CRISPR / Cas9介导的单等位基因缺失来研究小鼠胚胎干细胞功能增强
显微注射转基因的基因组,并在编辑Threespine刺鱼
腺病毒基因治疗对糖尿病性角膜病变:对伤口愈合和干细胞标记表达的人体器官培养角膜和角膜缘上皮细胞
BMPR-IB +快速分离脂肪来源的使用基质细胞在颅骨缺损愈合模型
新型乳腺脂肪移植技术可视化血管内皮干细胞的血管生成
用扫描电镜和全贴免疫荧光技术可视化小鼠胚胎中的节点和外型板
Read Article