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Journal / Bioengineering / O uso de um ensaio de β-lactamase-baseado…
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JoVE Journal Bioengineering
O uso de um ensaio de β-lactamase-baseado titulometria Biosensor para detectar interações biomoleculares
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O uso de um ensaio de β-lactamase-baseado titulometria Biosensor para detectar interações biomoleculares

Article DOI: 10.3791/55414-v • 08:06 min • February 1st, 2018
February 1st, 2018 •
*1, *1, 2, 1, 1, 1, 1, 3, 1, 4
1Life Science Department, University of Liège, 2Institute of Condensed Matter and Nanoscience, Catholic University of Louvain, 3Laboratory of Clinical Microbiology, Catholic University of Louvain, 4Biotechnology Department, CER Group
* These authors contributed equally

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Neste trabalho, relatamos um novo método para estudar as interações da proteína-proteína usando um biossensor titulometria, baseado na tecnologia de β-lactamases de híbrido. Este método baseia-se na liberação de prótons após hidrólise da β-lactamas.

Tags

Bioengenharia questão 132 β-lactamases tecnologia híbrida de proteína (BHP) titulometria biosensor nanobodies interações moleculares lisozima
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