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Utilizzando biosensori a base di enzimi misurare Tonic e Phasic glutammato in modelli murini di Alzheimer
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer’s Mouse Models
DOI:

10:46 min

May 03, 2017

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Chapters

  • 00:05Title
  • 00:50Coating the MEA
  • 01:40Electroplating the Electrode
  • 02:46Preparing the Solutions Needed for Calibration
  • 04:48Assemble the Micropipette
  • 05:46General Animal Surgery for MEA Recordings
  • 09:01Results: Extracellular Tonic and Potassium Chloride-evoked Release of Glutamate in Hippocampus
  • 10:18Conclusion

Summary

Automatic Translation

Qui, descriviamo l'installazione, software di navigazione, e l'analisi dei dati per un metodo spazialmente e temporalmente precisa misurazione toniche e fasiche cambiamenti glutammato extracellulare in vivo utilizzando matrici di microelettrodi enzimatico (MEA).

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