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Journal
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Biochemistry
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时间分辨电喷雾电离氢氘交换质谱用于研究蛋白质结构与动态
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JoVE Journal
Biochemistry
Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
Please note that all translations are automatically generated.
Click here for the English version.
时间分辨电喷雾电离氢氘交换质谱用于研究蛋白质结构与动态
DOI:
10.3791/55464-v
•
09:18 min
•
April 17, 2017
•
Cristina Lento
,
Shaolong Zhu
,
Kerene A. Brown
,
Ruth Knox
,
Peter Liuni
,
Derek J. Wilson
2,3
1
Department of Chemistry
,
York University
,
2
The Centre for Research in Mass Spectrometry
,
York University
,
3
The Centre for Research on Biomolecular Interactions
,
York University
Chapters
00:05
Title
01:12
Preparation of the Microfluidic Device
05:17
Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange
06:51
Results: Detection of Protein Structural Changes by Time-resolved ElectroSpray Ionization Hydrogen-deuterium Mass Spectrometry
08:10
Conclusion
Summary
Automatic Translation
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Automatic Translation
构象的灵活性在蛋白质功能的关键作用。在此,我们描述了使用耦合到氢 - 氘交换用于探测驱动功能在有序和无序的蛋白质的快速结构变化的时间分辨电喷雾电离质谱法。
Tags
Time-resolved Electrospray Ionization
Hydrogen-deuterium Exchange
Mass Spectrometry
Protein Structure
Protein Dynamics
Conformational Flexibility
Transient Protein Conformations
Loop Regions
Molten Globules
Intrinsically Disordered Proteins
Neurodegenerative Disorders
Protein Misfolding
Protein Aggregation
Enzyme Catalytic Turnover
Protein-protein Interactions
Protein-ligand Interactions
Continuous Flow
Time-resolved Kinetic Mixer
Orthogonal Mixing
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