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JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Test der Proteinkinase-Aktivität mit radioaktiv markiertem ATP
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Test der Proteinkinase-Aktivität mit radioaktiv markiertem ATP

Article DOI: 10.3791/55504
May 26th, 2017 •
1, 1, 1
1Department of Pharmacology, University of Texas Southwestern Medical Center

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May 26th, 2017

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Proteinkinasen sind hochentwickelte Signalisierungsenzyme und Gerüste, die für die inter- und intrazelluläre Signaltransduktion kritisch sind. Wir stellen ein Protokoll zur Messung der Kinaseaktivität durch die Verwendung von radioaktiv markiertem Adenosintriphosphat ([γ- 32 P] ATP) vor, ein zuverlässiges Verfahren zur Erleichterung der zellulären Signalregulation.

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