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Biochemistry

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JoVE Journal Biochemistry
Analys av proteinkinasaktivitet med radiomärkt ATP
Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy
Journal (Biochemistry)
Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy
Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry
Journal (Biochemistry)
Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry
 
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Analys av proteinkinasaktivitet med radiomärkt ATP

Article DOI: 10.3791/55504
May 26th, 2017 •
1, 1, 1
1Department of Pharmacology, University of Texas Southwestern Medical Center

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May 26th, 2017

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Proteinkinaser är högutvecklade signalerande enzymer och byggnadsställningar som är kritiska för inter- och intracellulär signaltransduktion. Vi presenterar ett protokoll för mätning av kinasaktivitet genom användning av radioaktivt märkt adenosintrifosfat ([γ-32P] ATP), en tillförlitlig metod som hjälper till att belysa cellsignalreglering.

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