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Four-Color Fluorescence Immunohistochemistry af T-Cell Subpopulations i Archival Formalin-Fixed, Paraffin-Embedded Human Oropharyngeal Squamous Cell Carcinoma Samples
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Cancer Research
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JoVE Journal Cancer Research
Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples

Four-Color Fluorescence Immunohistochemistry af T-Cell Subpopulations i Archival Formalin-Fixed, Paraffin-Embedded Human Oropharyngeal Squamous Cell Carcinoma Samples

DOI:
10.3791/55589-v

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07:43 min

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July 29, 2017

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, ,
1Department of Pathology,Leiden University Medical Center, 2Department of Otorhinolaryngology and Head and Neck Surgery,Erasmus University Medical Center, 3Center for Gynecological Oncology Amsterdam,VU Medical Center

Chapters

  • 00:05Title
  • 01:04Antigen Retrieval
  • 02:16Tissue Staining and Analysis
  • 05:12Results: Representative Formalin-fixed, Paraffin-embedded Oropharyngeal Cancer Tissue Staining and Analysis
  • 06:42Conclusion

Summary

Automatic Translation

Automatic Translation

Multiparameterfluorescensimmunohistokemi kan anvendes til at vurdere antallet, den relative fordeling og lokalisering af immuncellepopulationer i tumormikromiljøet. Dette manuskript beskriver brugen af ​​denne teknik til analyse af T-celle subpopulationer i orofaryngealkræft.

Tags

ImmunohistochemistryT-cell SubpopulationsOropharyngeal Squamous Cell CarcinomaFormalin-fixed Paraffin-embeddedMulticolorBiomarkersImmune CellsCancer TherapyPatient StratificationImmune Therapies

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