Journal
/
Developmental Biology
/
在奥运电击鸡听觉脑干中
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
In Ovo Electroporation in the Chicken Auditory Brainstem
Please note that all translations are automatically generated. Click here for the English version.

在奥运电击鸡听觉脑干中

DOI:
10.3791/55628-v

•

10:14 min

•

June 09, 2017

•

, ,
1Roxelyn and Richard Pepper Department of Communication Sciences and Disorders,Northwestern University, 2Knowles Hearing Research Center,Northwestern University, 3Department of Neurobiology,Northwestern University

Chapters

  • 00:05Title
  • 00:54Plasmid Preparation
  • 02:01Egg Handling and Windowing
  • 04:15Plasmid Injection
  • 06:11Electroporation
  • 08:03Results: Spatial Protein Expression After In Ovo Electroporation
  • 09:19Conclusion

Summary

Automatic Translation

Automatic Translation

鸟类和哺乳动物的听觉脑干神经元专门用于快速神经编码，这是正常听力功能的基本过程。这些神经元来自胚胎后脑的不同前体。我们提出利用电穿孔技术在鸡胚后脑中表达基因以研究听觉发育过程中的基因功能。

Tags

In Ovo ElectroporationChicken Auditory BrainstemGene ExpressionAuditory Neuron DevelopmentPlasmid SolutionMicropipette PullerPicospritzerHamburger-Hamilton StageEgg IncubationEggshell Membrane RemovalAlbumin Withdrawal

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

在小鸡视网膜有效的基因转移的原代细胞培养研究：<em>前OVO</em>电穿孔法

斑马鱼幼虫骨骼肌诚信与伊文思蓝分析

鸡胚全视网膜外植体电穿孔和化学试剂处理

基因转移到鸡听觉器官通过<em>在卵</em>微电

一本小说<em>防爆大毛</em>带技术来改变心内血流动力学在鸡胚胎系统

胚胎显微注射和电的脊索动物<em>玻璃海鞘</em

成人果蝇脑内蘑菇体和感光神经元的解剖和荧光染色

三维脊髓前庭和听觉感官器官的培养

用扫描电镜和全贴免疫荧光技术可视化小鼠胚胎中的节点和外型板

奥多纳塔电介质介质RNA干扰法

Read Article