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Sialik asit Nkullanarak metabolik Glycoengineering-asil-Mannosamines modifiye
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1Max von Pettenkofer-Institut & Genzentrum, Virologie, Nationales Referenzzentrum für Retroviren, Medizinische Fakultät,LMU München, 2Institut für Laboratoriumsmedizin, klinische Chemie und Pathobiochemie,Charité – Universitätsmedizin Berlin, 3Institut für Physiologische Chemie,Martin-Luther-Universität Halle-Wittenberg
Chapters
- 00:05Title
- 01:02Synthesis of ManNProp and Related N-acyl-modefied Mannosamines
- 02:26Cell Culture and Cell Lysis for HPLC Analysis
- 04:28Separation of the Membrane Fraction and Acid Hydrolysis
- 05:27Fluorescent Labeling, HPLC, and Mass Spetrometry Analysis
- 06:55Western Blot Analysis of Polysialic Acid in Kelly Neuroblastoma Cells
- 09:00Results: Modulating Expression of Sialic Acid on Cell Surfaces Using N-Side Chain Modified Mannosamines
- 10:40Conclusion
Sialik asit glycoconjugates içinde bulunan tipik bir yaşam ünitesi var. Moleküler ve hücresel etkileşimlerin bir bolluk ilgilenmektedir. Burada hücre yüzey sialik asit ifadesi N- acetylmannosamine türevleri ile metabolik glycoengineering kullanarak değiştirmek için bir yöntem mevcut.
