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Sialik asit Nkullanarak metabolik Glycoengineering-asil-Mannosamines modifiye
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Metabolic Glycoengineering of Sialic Acid Using N-acyl-modified Mannosamines
DOI:

12:06 min

November 25, 2017

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Chapters

  • 00:05Title
  • 01:02Synthesis of ManNProp and Related N-acyl-modefied Mannosamines
  • 02:26Cell Culture and Cell Lysis for HPLC Analysis
  • 04:28Separation of the Membrane Fraction and Acid Hydrolysis
  • 05:27Fluorescent Labeling, HPLC, and Mass Spetrometry Analysis
  • 06:55Western Blot Analysis of Polysialic Acid in Kelly Neuroblastoma Cells
  • 09:00Results: Modulating Expression of Sialic Acid on Cell Surfaces Using N-Side Chain Modified Mannosamines
  • 10:40Conclusion

Summary

Automatic Translation

Sialik asit glycoconjugates içinde bulunan tipik bir yaşam ünitesi var. Moleküler ve hücresel etkileşimlerin bir bolluk ilgilenmektedir. Burada hücre yüzey sialik asit ifadesi N- acetylmannosamine türevleri ile metabolik glycoengineering kullanarak değiştirmek için bir yöntem mevcut.

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